Antitumour Activity of Different Bioactive Compounds from Hemolymph and Mucus of Mollusca against Human Urinary Bladder Cancer Cell Lines

Authors

  • Maria Nikolova Department of Pharmacology, Pharmacotherapy and Toxicology Faculty of Pharmacy, Medical University of Sofia
  • Spiro Konstantinov Department of Pharmacology, Pharmacotherapy and Toxicology Faculty of Pharmacy, Medical University of Sofia
  • Lyudmila Velkova Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences
  • Dimiter Kaynarov Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences
  • Aleksandar Dolashki Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences
  • Pavlina Dolashka Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences

DOI:

https://doi.org/10.7546/CRABS.2022.05.13

Keywords:

urothelial carcinoma, molluscan, hemocyanins, antitumour activity, nuclear changes

Abstract

In this study, we isolated different bioactive compounds and fractions from Rapana venosa (RvH), Helix aspersa (HaH) and Helix lucorum (HlH) and then we evaluated their in vitro antitumour activity on CAL-29 and T- 24 cell lines (transitional cell carcinoma). Also, we performed fluorescence staining with propidium iodide to assess nuclear changes caused by molluscan extracts. Briefly, our results have demonstrated antineoplastic activity of: subunit βc-HaH of H. aspersa hemocyanins to CAL-29 cell line, subunit βc-HaH and subunit RvH II of R. venosa hemocyanin against T-24 cells. Also, fractions from R. venosa hemolymph exerted significant cytotoxic activity against CAL- 29 cells. Furthermore, fluorescence microscopy results demonstrated chromatin condensation and nuclear morphological changes which are typical for apoptosis induction. We assume that the observed death of T-24 and CAL-29 cells is due to the interaction between the oligosaccharide structures of the proteins on the tumour cell membranes and the hemolymph from R. venosa, active components isolated from hemolymph of R. venosa, H. aspersa and H. lucorum, as well as H. aspersa mucus.

Author Biographies

Maria Nikolova, Department of Pharmacology, Pharmacotherapy and Toxicology Faculty of Pharmacy, Medical University of Sofia

Mailing Address:
Department of Pharmacology,
Pharmacotherapy and Toxicology
Faculty of Pharmacy,
Medical University of Sofia
1 St. Georgi Sofiiski St
1431 Sofia, Bulgaria

E-mail: maria.nikolova.sofia@abv.bg

Spiro Konstantinov, Department of Pharmacology, Pharmacotherapy and Toxicology Faculty of Pharmacy, Medical University of Sofia

Mailing Address:
Department of Pharmacology,
Pharmacotherapy and Toxicology
Faculty of Pharmacy,
Medical University of Sofia
1 St. Georgi Sofiiski St
1431 Sofia, Bulgaria

E-mail: konstantinov.spiromihaylov@gmail.com

Lyudmila Velkova, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences

Mailing Address:
Institute of Organic Chemistry
with Centre of Phytochemistry,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 9
1113 Sofia, Bulgaria

E-mail: lyudmila_velkova@abv.bg

Dimiter Kaynarov, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences

Mailing Address:
Institute of Organic Chemistry
with Centre of Phytochemistry,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 9
1113 Sofia, Bulgaria

E-mail: mitkokaynarov@abv.bg

Aleksandar Dolashki, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences

Mailing Address:
Institute of Organic Chemistry
with Centre of Phytochemistry,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 9
1113 Sofia, Bulgaria

E-mail: adolashki@yahoo.com

Pavlina Dolashka, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences

Mailing Address:
Institute of Organic Chemistry
with Centre of Phytochemistry,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 9
1113 Sofia, Bulgaria

E-mail: pda54@abv.bg

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Published

31-05-2022

How to Cite

[1]
M. Nikolova, S. Konstantinov, L. Velkova, D. Kaynarov, A. Dolashki, and P. Dolashka, “Antitumour Activity of Different Bioactive Compounds from Hemolymph and Mucus of Mollusca against Human Urinary Bladder Cancer Cell Lines”, C. R. Acad. Bulg. Sci. , vol. 75, no. 5, pp. 726–736, May 2022.

Issue

Section

Medicine