Callus via Shoot Organogenesis and Plant Regeneration of Stevia rebaudiana Bertoni
Keywords:stevia, callus from leaf and stem, indirect organogenesis
Stevia rebaudiana Bertoni is important medicinal plant which is native between southern Brazil and northern Paraguay. A reliable protocol for shoot organogenesis and regeneration was developed for an antidiabetic medicinal plant species. Regeneration via indirect shoot organogenesis was established from two types of explant (leaf and stem) on MS medium  with different concentrations and combinations of plant growth regulators (PGR) such as 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and gibberellic acid (GA3) for inducing organogenic callus of S. rebaudiana. In the present investigation the highest percentage of response was shown by 90% and 80% callogenesis from leaf and stem explants on MS medium supplemented with 2.0 mg/l 2,4-D + 0.5 mg/l BAP + 0.5 mg/l GA3 (MSC3) was observed. The high frequency of regenerants was observed from S. rebaudiana leaves at 0.5 mg/l 2,4-D + 2.0 mg/l BAP + 0.5 mg/l GA3 (MSR3). Optimal shoot multiplication and rooting were obtained on 2.0 mg/l BAP and 0.1 mg/l indole- 3-butyric acid (IBA), respectively, followed by successful acclimatization of regenerants (90% survival) to greenhouse condition. The purpose of this study was to establish a reliable S. rebaudiana regeneration protocol that can be used to facilitate the cultivation, propagation and planting of this plant species.
How to Cite
LicenseCopyright (c) 2022 Proceedings of the Bulgarian Academy of Sciences
Copyright (c) 2022 Proceedings of the Bulgarian Academy of Sciences
Copyright is subject to the protection of the Bulgarian Copyright and Associated Rights Act. The copyright holder of all articles on this site is Proceedings of the Bulgarian Academy of Sciences. If you want to reuse any part of the content, please, contact us.