The Influence of Microcystin-LR and Microcystin-LR-Producing Trichormus variabilis (Cyanobacteria) on Green Microalgae in Laboratory Conditions

Authors

  • Tamara Petronijevic Faculty of Sciences and Mathematics, University of Niš, Serbia
  • Djuradj Milosevic Faculty of Sciences and Mathematics, University of Niš, Serbia
  • Ivana Kostic Kokic Faculty of Sciences and Mathematics, University of Niš, Serbia
  • Milica Stojkovic Piperac Faculty of Sciences and Mathematics, University of Niš, Serbia
  • Nikola Stankovic Faculty of Sciences and Mathematics, University of Niš, Serbia and Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Germany

DOI:

https://doi.org/10.7546/CRABS.2024.06.07

Keywords:

microcystins, chlorophyll a, growth, HPLC, co-culture

Abstract

The most studied cyanobacterial toxins are microcystins (MCs), of which microcystin-LR (MC-LR) is the most common, with the most experimentally documented toxicity to aquatic species. The aim of the present study is to examine the influence of an ecologically relevant MC-LR concentration (10 µg L-1) on green microalgae (Chlorella sp., Scenedesmus sp., Coelastrum sp.) by determining the concentration of chlorophyll a. In addition, the influence of the Trichormus variabilis was monitored, characterized as MC-LR producer, on the growth of green microalgae, using the co-cultivation method. MC-LR was detected, quantified, and identified in the methanolic extract of T. variabilis using the high-performance liquid chromatography (HPLC). The study revealed that MC-LR significantly impacted green microalgae growth causing stimulation. In contrast, T. variabilis in co-culture exhibited only an inhibitory effect on the growth. The study revealed the allelopathic effect of environmentally relevant microcystin-LR concentration on green microalgae.

Author Biographies

Tamara Petronijevic, Faculty of Sciences and Mathematics, University of Niš, Serbia

Mailing Address:
Department of Biology and Ecology,
Faculty of Sciences and Mathematics,
University of Niš
Višegradska 33
Niš 18000, Serbia

Email: tamara.petronijevic@pmf.edu.rs

Djuradj Milosevic, Faculty of Sciences and Mathematics, University of Niš, Serbia

Mailing Address:
Department of Biology and Ecology,
Faculty of Sciences and Mathematics,
University of Niš
Višegradska 33
Niš 18000, Serbia

Email: djuradj.milosevic@gmail.com

Ivana Kostic Kokic, Faculty of Sciences and Mathematics, University of Niš, Serbia

Mailing Address:
Department of Chemistry,
Faculty of Sciences and Mathematics,
University of Niš
Višegradska 33
Niš 18000, Serbia

Email: ivana.kostic83@gmail.com

Milica Stojkovic Piperac, Faculty of Sciences and Mathematics, University of Niš, Serbia

Mailing Address:
Department of Biology and Ecology,
Faculty of Sciences and Mathematics,
University of Niš
Višegradska 33
Niš 18000, Serbia

Email: milicastojkovic83@gmail.com

Nikola Stankovic, Faculty of Sciences and Mathematics, University of Niš, Serbia and Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Germany

Mailing Address:
Department of Biology and Ecology,
Faculty of Sciences and Mathematics,
University of Niš
Višegradska 33
Niš 18000, Serbia
and
Leibniz-Institute of Freshwater Ecology and Inland Fisheries,
Muggelseedamm 301
12587 Berlin, Germany

Email: nikola.stankovic@pmf.edu.rs

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Published

30-06-2024

How to Cite

[1]
T. Petronijevic, D. Milosevic, I. Kokic, M. Piperac, and N. Stankovic, “The Influence of Microcystin-LR and Microcystin-LR-Producing Trichormus variabilis (Cyanobacteria) on Green Microalgae in Laboratory Conditions”, C. R. Acad. Bulg. Sci. , vol. 77, no. 6, pp. 839–848, Jun. 2024.

Issue

Section

Biology