Morphometric Changes of the Myenteric Plexus in the Colon of the Mice-D-Galactose Ageing Model

Authors

  • Nikolay Genov Department of Anatomy, Faculty of Medicine, Trakia University, Bulgaria
  • Nikola Tomov Institute of Anatomy, University of Bern, Switzerland
  • Nikolay Dimitrov Department of Anatomy, Faculty of Medicine, Trakia University, Bulgaria
  • Todor Kirov Department of Anatomy and Histology, Faculty of Medicine, Medical University of Sofia, Bulgaria
  • Lubomir Petrov Institute of Neurobiology, Bulgarian Academy of Sciences and National Sports Academy “Vassil Levski”, Bulgaria
  • Elina Tsvetanova Institute of Neurobiology, Bulgarian Academy of Sciences
  • Almira Georgieva Institute of Neurobiology, Bulgarian Academy of Sciences
  • Albena Alexandrova Institute of Neurobiology, Bulgarian Academy of Sciences and National Sports Academy “Vassil Levski”, Bulgaria
  • Nikolai Lazarov Department of Anatomy and Histology, Faculty of Medicine, Medical University of Sofia and Institute of Neurobiology, Bulgarian Academy of Sciences
  • Dimitrinka Atanasova Department of Anatomy, Faculty of Medicine, Trakia University and Institute of Neurobiology, Bulgarian Academy of Sciences

DOI:

https://doi.org/10.7546/CRABS.2024.04.13

Keywords:

myenteric plexus, D-galactose, ageing model, colon, mice

Abstract

Ageing-related diseases are paramount for understanding the socioeconomic impact of the postmodern society. Neuronal degeneration is a known phenomenon in ageing. The D-galactose model of accelerated ageing is widely used in age-associated neuronal cell death because of the oxidative stress it induces, which correlates to reduced cognitive performance and robustly detectable neuronal shrinkage in the CNS. This study aims at demonstrating the morphological changes of neurons of the myenteric plexus in mice treated with D-galactose. D-galactose was given orally with drinking water, resulting in an average dose of 500 mg/kg daily for six weeks. In the D-galactose accelerated ageing model, a significant size reduction of the neuronal perikarya of the myenteric plexus was observed all along the large intestine. The average soma area at the caecum in the control group was 305 µm2, which was reduced by almost 20% in the ageing model. In contrast, at the level of the proximal colon in the D-galactose ageing model, the average area of the neuronal soma was 280 µm2, a reduction of 30%. The most significant reduction has been observed at the level of the distal colon, where the neuronal soma was reduced by 40%. There is a significant reduction of the area of the neuronal bodies in the myenteric plexus among different parts of the mice's large intestine in the accelerated ageing model. It can be inferred that D-galactose-induced morphological changes in the myenteric plexus may be related to the increased gastrointestinal motility dysfunction with ageing.

Author Biographies

Nikolay Genov, Department of Anatomy, Faculty of Medicine, Trakia University, Bulgaria

Mailing Address:
Department of Anatomy,
Faculty of Medicine,
Trakia University
11 Armejska St, 6000 Stara Zagora, Bulgaria

E-mail: nikolay.genov@trakia-uni.bg

Nikola Tomov, Institute of Anatomy, University of Bern, Switzerland

Mailing Address:
Institute of Anatomy,
University of Bern
Baltzerstrasse 2, 3012 Bern, Switzerland

E-mail: nikola.tomov@unibe.ch

Nikolay Dimitrov, Department of Anatomy, Faculty of Medicine, Trakia University, Bulgaria

Mailing Address:
Department of Anatomy,
Faculty of Medicine,
Trakia University,
11 Armejska St, 6000 Stara Zagora, Bulgaria

E-mail: nikolay.dimitrov@trakia-uni.bg

Todor Kirov, Department of Anatomy and Histology, Faculty of Medicine, Medical University of Sofia, Bulgaria

Mailing Address:
Department of Anatomy and Histology,
Faculty of Medicine,
Medical University of Sofia
1 St. G. Sofiiski St, 1431 Sofia, Bulgaria

E-mail: tkirov@medfac.mu-sofia.bg

Lubomir Petrov, Institute of Neurobiology, Bulgarian Academy of Sciences and National Sports Academy “Vassil Levski”, Bulgaria

Mailing Address:
Institute of Neurobiology,
Bulgarian Academy of Sciences,
Akad. G. Bonchev St, Bl. 23,
1113 Sofia, Bulgaria

and

National Sports Academy “Vassil Levski”,
21 Akad. Stefan Mladenov St,
1700 Sofia, Bulgaria

E-mail: dr.lubomir.petrov@gmail.com

Elina Tsvetanova, Institute of Neurobiology, Bulgarian Academy of Sciences

Mailing Address:
Institute of Neurobiology,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 23,
1113 Sofia, Bulgaria

E-mail: e.tsvetanova@bio.bas.bg

Almira Georgieva, Institute of Neurobiology, Bulgarian Academy of Sciences

Mailing Address:
Institute of Neurobiology,
Bulgarian Academy of Sciences,
Akad. G. Bonchev St, Bl. 23,
1113 Sofia, Bulgaria

E-mail: a.georgieva@inb.bas.bg

Albena Alexandrova, Institute of Neurobiology, Bulgarian Academy of Sciences and National Sports Academy “Vassil Levski”, Bulgaria

Mailing Address:
Institute of Neurobiology,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 23,
1113 Sofia, Bulgaria

and

National Sports Academy “Vassil Levski”,
21 Akad. Stefan Mladenov St,
1700 Sofia, Bulgaria

E-mail: a.alexandrova@inb.bas.bg

Nikolai Lazarov, Department of Anatomy and Histology, Faculty of Medicine, Medical University of Sofia and Institute of Neurobiology, Bulgarian Academy of Sciences

Mailing Address:
Department of Anatomy and Histology,
Faculty of Medicine,
Medical University of Sofia
1 St. G. Sofiiski St, 1431 Sofia, Bulgaria

and

Institute of Neurobiology,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 23,
1113 Sofia, Bulgaria

E-mail: nlazarov@medfac.mu-sofia.bg

Dimitrinka Atanasova, Department of Anatomy, Faculty of Medicine, Trakia University and Institute of Neurobiology, Bulgarian Academy of Sciences

Mailing Addresses:
Department of Anatomy,
Faculty of Medicine,
Trakia University
11 Armejska St, 6000 Stara Zagora, Bulgaria

and

Institute of Neurobiology,
Bulgarian Academy of Sciences,
Akad. G. Bonchev St, Bl. 23,
1113 Sofia, Bulgaria

E-mails: dimitrinka.atanasova-dimitrova@trakia-uni.bg,
d.atanasova@inb.bas.bg

Downloads

Published

26-04-2024

How to Cite

[1]
N. Genov, “Morphometric Changes of the Myenteric Plexus in the Colon of the Mice-D-Galactose Ageing Model”, C. R. Acad. Bulg. Sci. , vol. 77, no. 4, pp. 576–583, Apr. 2024.

Issue

Section

Medicine