In Vitro Evaluation of Biocompatibility of Two Dental Alloys on Stem Cells from Apical Papilla – a Comparative Study

Authors

  • Marya Dencheva Faculty of Dental Medicine, Medical University of Sofia, Bulgaria
  • Maya Lyapina Medical College “J. Filaretova”, Medical University of Sofia, Bulgaria

DOI:

https://doi.org/10.7546/CRABS.2024.04.06

Keywords:

dental materials, dental alloys, corrosion, haptens

Abstract

In the present study we performed a comparative in vitro evaluation of biocompatibility of two nickel-containing dental alloys produced by two different methods – conventional and powder metallurgy. Stem cells from apical papilla (SCAPs) were isolated, cultured and incubated in 6% solution of alloys powders in DMSO for 48 hours and further examined for apoptosis, proliferation and cellular metabolism. Our findings suggest that the alloys produced by the traditional method (Crystalloy N) release more electrically charged products of electrochemical corrosion, resulting in increased ROS production in SCAPs, increased percentage of apoptoic cells, decreased proliferative potential and tend to increase antioxidant enzymatic defence. No allergic pro-inflammatory effect of the metal alloys on stem cell cultures was confirmed.

Author Biographies

Marya Dencheva, Faculty of Dental Medicine, Medical University of Sofia, Bulgaria

Mailing Address:
Faculty of Dental Medicine,
Medical University of Sofia,
1 St. Georgi Sofiiski St,
1431 Sofia, Bulgaria

E-mail: dr.maria.dencheva@gmail.com

Maya Lyapina, Medical College “J. Filaretova”, Medical University of Sofia, Bulgaria

Mailing Address:
Medical College “J. Filaretova”,
Medical University of Sofia,
3 J. Filaretova St, 1606 Sofia, Bulgaria

E-mail: saly_grigory@abv.bg

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Published

26-04-2024

How to Cite

[1]
M. Dencheva and M. Lyapina, “In Vitro Evaluation of Biocompatibility of Two Dental Alloys on Stem Cells from Apical Papilla – a Comparative Study”, C. R. Acad. Bulg. Sci. , vol. 77, no. 4, pp. 520–527, Apr. 2024.

Issue

Section

Biology