In Vitro Evaluation of Biocompatibility of Two Dental Alloys on Stem Cells from Apical Papilla – a Comparative Study

Abstract

In the present study we performed a comparative in vitro evaluation of biocompatibility of two nickel-containing dental alloys produced by two different methods – conventional and powder metallurgy. Stem cells from apical papilla (SCAPs) were isolated, cultured and incubated in 6% solution of alloys powders in DMSO for 48 hours and further examined for apoptosis, proliferation and cellular metabolism. Our findings suggest that the alloys produced by the traditional method (Crystalloy N) release more electrically charged products of electrochemical corrosion, resulting in increased ROS production in SCAPs, increased percentage of apoptoic cells, decreased proliferative potential and tend to increase antioxidant enzymatic defence. No allergic pro-inflammatory effect of the metal alloys on stem cell cultures was confirmed.