In Vitro Wound-healing Activity of Ziziphus jujuba and Its Anti-inflammatory Effects in Rats

Authors

  • Insaf Fatima Zohra Mansour Oran 1 Ahmed Benbella University, Faculty of Life and Natural Sciences, Algeria
  • Cennet Ozay Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Izmir Katip Celebi University, Turkey
  • Fadime Aydın Kose Department of Biochemistry, Faculty of Pharmacy, Izmir Katip Celebi University, Turkey
  • Soraya Rahmanı Department of Biotechnology and Agronomic Sciences, Faculty of Science of Nature and Life, Hassiba Ben Bouali University, Algeria
  • Omar Kharoubı Oran 1 Ahmed Benbella University, Faculty of Life and Natural Sciences, Algeria

DOI:

https://doi.org/10.7546/CRABS.2024.01.14

Keywords:

Ziziphus jujuba Mill., jujube, wound healing, paw edema, anti-inflammatory

Abstract

Jujube (Ziziphus jujuba) has a long history of use in traditional medicine. It has many pharmacological properties, including neuroprotective, antioxidative, hepatoprotective, anti-cancer, anti-inflammatory, and immunomodulatory. Thus, this study aimed to evaluate the anti-inflammatory activity of the ethanolic extract of Ziziphus (EEZ) fruit and its wound-healing effect. The anti-inflammatory activity of EEZ was assessed in vivo by carrageenan-induced paw edema test. NIH-3T3 mouse embryonic fibroblast cells were used to evaluate the wound healing effect of EEZ. The effect of the extract on wound-healing was examined by in vitro scratch test. Also, the level of C-reactive protein (CRP) in the serum of rats was determined using the CRP latex test kit. The ethanol extract of jujube fruit induced wound closure in NIH-3T3 fibroblast cells for 18 h (88.5%) at 1 μ\umu g/mL concentration. Also, EEZ significantly inhibited carrageenan-induced edema in rat paws. However, a statistically significant decrease in CRP levels was observed in rats pre-treated with EEZ. As a result, Z. jujuba fruit extract had anti-inflammatory and wound healing effects, which might be strongly related to its phytochemical composition.

Author Biographies

Insaf Fatima Zohra Mansour, Oran 1 Ahmed Benbella University, Faculty of Life and Natural Sciences, Algeria

Mailing Address:
Oran 1 Ahmed Benbella University,
Faculty of Life and Natural Sciences,
Laboratory of Experimental Biotoxicology, Biodepollution and Phytoremediation,
Oran 31000, Algeria

E-mail: mansour.insaf@univ-oran1.dz

Cennet Ozay, Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Izmir Katip Celebi University, Turkey

Mailing Address:
Department of Basic Pharmaceutical Sciences,
Faculty of Pharmacy,
Izmir Katip Celebi University
Izmir 35620, Turkey

E-mail: cennet.ozay@ikcu.edu.tr

Fadime Aydın Kose, Department of Biochemistry, Faculty of Pharmacy, Izmir Katip Celebi University, Turkey

Mailing Address:
Department of Biochemistry,
Faculty of Pharmacy,
Izmir Katip Celebi University
Izmir 35620, Turkey

E-mail: fadime.aydin.kose@ikcu.edu.tr

Soraya Rahmanı, Department of Biotechnology and Agronomic Sciences, Faculty of Science of Nature and Life, Hassiba Ben Bouali University, Algeria

Mailing Address:
Department of Biotechnology and Agronomic Sciences,
Faculty of Science of Nature and Life,
Hassiba Ben Bouali University
Chlef 16000, Algeria

E-mail: rahmanisoraya11@gmail.com

Omar Kharoubı, Oran 1 Ahmed Benbella University, Faculty of Life and Natural Sciences, Algeria

Mailing Address:
Oran 1 Ahmed Benbella University,
Faculty of Life and Natural Sciences,
Laboratory of Experimental Biotoxicology, Biodepollution and Phytoremediation,
Oran 31000, Algeria

E-mail: e-mail: omarkharoubi@gmail.com

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Published

29-01-2024

How to Cite

[1]
I. Mansour, C. Ozay, F. Kose, S. Rahmanı, and O. Kharoubı, “In Vitro Wound-healing Activity of Ziziphus jujuba and Its Anti-inflammatory Effects in Rats”, C. R. Acad. Bulg. Sci. , vol. 77, no. 1, pp. 118–126, Jan. 2024.

Issue

Section

Medicine