Identification of Citrobacter freundii from Skin Wounds in Goldfish (Carassius auratus) and Determination of Antibacterial Susceptibility

Authors

  • Volkan Özavci Department of Microbiology, Faculty of Veterinary Medicine, Dokuz Eyl¨ul University, Turkey
  • Hafize Tu˘gba Y¨uksel Dolgun Department of Microbiology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Turkey
  • S¨ukr¨u Kirkan Department of Microbiology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Turkey

DOI:

https://doi.org/10.7546/CRABS.2023.06.11

Keywords:

goldfish, Citrobacter freundii, identification, sequence, antibiogram

Abstract

Citrobacter freundii is a zoonotic pathogen that causes skin injuries and high mortality rates in fish. This study aims to determine the presence of C. freundii and antibiotic susceptibility in 50 goldfish (Carassius auratus) skin wounds with local bleeding between the ages of 8–18 months in three regions (A, B, and C) located in the West of Turkey. C. freundii (22/50; 44%) strains were isolated from wound swab samples by phenotypic and genotypic methods. C. freundii isolates were positive for H2S, mannitol, and indole, and were negative for the gas formation and urea. All C. freundii isolates showed 96% similarity to C. freundii strain BAO2749 according to the Sanger sequence method. Isolates were also resistant to ampicillin at 57.09% and susceptible to cefoperazone and sulfamethoxazole-trimethoprim by 100%. Statistical analyses were evaluated at a confidence interval of 95%. A p value < 0.05 was considered statistically significant. In conclusion, C. freundii isolates can play a significant role in the pathophysiology of infections in goldfish (Carassius auratus). It can also cause commercial losses in businesses. For this reason, it will be beneficial to control C. freundii pathogenicity in aquarium fish with prophylactic applications to improve product quality and treat diseases in ornamental fish farming. 

Author Biographies

Volkan Özavci, Department of Microbiology, Faculty of Veterinary Medicine, Dokuz Eyl¨ul University, Turkey

Mailing Address:
Department of Microbiology,
Faculty of Veterinary Medicine,
Dokuz Eyl¨ul University
Kiraz/Izmir, 35890, T¨urkiye

E-mail: volkan.ozavci@deu.edu.tr

Hafize Tu˘gba Y¨uksel Dolgun, Department of Microbiology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Turkey

Mailing Address:
Department of Microbiology,
Faculty of Veterinary Medicine,
Aydin Adnan Menderes University
Efeler/Aydın, 09010, T¨urkiye

E-mail: tugba.yuksel@adu.edu.tr

S¨ukr¨u Kirkan, Department of Microbiology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Turkey

Mailing Address:
Department of Microbiology,
Faculty of Veterinary Medicine,
Aydin Adnan Menderes University
Efeler/Aydın, 09010, T¨urkiye

E-mail: skirkan@adu.edu.tr

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Published

29-06-2023

How to Cite

[1]
V. Özavci, H. Dolgun, and S. Kirkan, “Identification of Citrobacter freundii from Skin Wounds in Goldfish (Carassius auratus) and Determination of Antibacterial Susceptibility”, C. R. Acad. Bulg. Sci. , vol. 76, no. 6, pp. 911–918, Jun. 2023.

Issue

Section

Biology