Higher Expression Levels of miR-1246 in Advanced Laryngeal Carcinoma Are Associated with Elevated Risk of Locoregional Metastasis

Authors

  • Gergana Stancheva Molecular Medicine Center, Medical University-Sofia, Bulgaria
  • Silva Kyurkchiyan Molecular Medicine Center, Medical University-Sofia, Bulgaria
  • Veronika Petkova Molecular Medicine Center, Medical University-Sofia
  • Tzvetomir Marinov Department of Anesthesiology and Intensive Care, Medical University-Sofia, University Hospital “Tsaritsa Yoanna – ISUL”, Bulgaria
  • Maya Belitova Department of Anesthesiology and Intensive Care, Medical University-Sofia, University Hospital “Tsaritsa Yoanna – ISUL”, Bulgaria
  • Sylvia Skelina Department of Neurology, Medical University-Sofia, Bulgaria
  • Radka Kaneva Molecular Medicine Center, Medical University-Sofia, Bulgaria
  • Diana Popova Department of ENT, Medical University-Sofia, Bulgaria
  • Todor M. Popov Department of ENT, Medical University-Sofia, Bulgaria

DOI:

https://doi.org/10.7546/CRABS.2023.03.16

Keywords:

laryngeal carcinoma, head and neck cancer, field cancerization, locoregional metastasis survival, miR-1246

Abstract

Worldwide, laryngeal squamous cell carcinoma (LSCC) is the second most common head and neck cancer (HNC). Advanced forms of LSCC, especially HPV-negative types, frequently involve locoregional lymph node metastasis, which are associated with treatment failure. Despite recent development and improvement in treatment protocols [1], still advanced LSCC is a global oncological burden, associated with challenges and complications. The study group included 60 cases of predominantly HPV-negative tumours with all patients having history of long-term smoking. We found that miR-1246 was significantly dysregulated in advanced LSCC – almost 72% of the patients in the study group showed overexpression of miR-1246 in tumour tissue above relative quantity of 2 (RQ > 2). Additionally, miR-1246 showed significant heterogeneity in expression levels between tumour depth and surface and moreover higher expression levels of the molecule correlated with higher chance of locoregional metastasis. In conclusion, we explored the expression levels of miR-1246 in advanced LSCC and adjacent normal tissue, revealing new tumour heterogeneity characteristic. We demonstrated potential association between miR-1246 expression levels and presence of locoregional lymph nodal metastasis.

Author Biographies

Gergana Stancheva, Molecular Medicine Center, Medical University-Sofia, Bulgaria

Mailing Address:
Molecular Medicine Center,
Medical University-Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: g.stancheva@medfac.mu-sofia.bg

Silva Kyurkchiyan, Molecular Medicine Center, Medical University-Sofia, Bulgaria

Mailing Address:
Molecular Medicine Center,
Medical University-Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: s.kyurkchiyan@medfac.mu-sofia.bg

Veronika Petkova, Molecular Medicine Center, Medical University-Sofia

Mailing Address:
Molecular Medicine Center,
Medical University-Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: v.petkova@medfac.mu-sofia.bg

Tzvetomir Marinov, Department of Anesthesiology and Intensive Care, Medical University-Sofia, University Hospital “Tsaritsa Yoanna – ISUL”, Bulgaria

Mailing Address:
Department of Anesthesiology and Intensive Care,
Medical University-Sofia,
University Hospital “Tsaritsa Yoanna – ISUL”
8 Byalo More St
1527 Sofia, Bulgaria

E-mail: ts.marinov@medfac.mu-sofia.bg

Maya Belitova, Department of Anesthesiology and Intensive Care, Medical University-Sofia, University Hospital “Tsaritsa Yoanna – ISUL”, Bulgaria

Mailing Address:
Department of Anesthesiology and Intensive Care,
Medical University-Sofia,
University Hospital “Tsaritsa Yoanna – ISUL”
8 Byalo More St
1527 Sofia, Bulgaria

E-mail: mbelitova@medfac.mu-sofia.bg,
belitova@mail.bg

Sylvia Skelina, Department of Neurology, Medical University-Sofia, Bulgaria

Mailing Address:
Department of Neurology,
Medical University-Sofia
1 St. G. Sofiiski Blvd
1431 Sofia, Bulgaria

E-mail: s.skelina@medfac.mu-sofia.bg

Radka Kaneva, Molecular Medicine Center, Medical University-Sofia, Bulgaria

Mailing Address:
Molecular Medicine Center,
Medical University-Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: r.kaneva@medfac.mu-sofia.bg

Diana Popova, Department of ENT, Medical University-Sofia, Bulgaria

Mailing Address:
Department of ENT,
Medical University-Sofia
10 Adriana Budevska St
1463 Sofia, Bulgaria

E-mail: d.popova@medfac.mu-sofia.bg

Todor M. Popov, Department of ENT, Medical University-Sofia, Bulgaria

Mailing Address:
Department of ENT,
Medical University-Sofia
10 Adriana Budevska St
1463 Sofia, Bulgaria

E-mail: t.popov@medfac.mu-sofia.bg

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Published

27-03-2023

How to Cite

[1]
G. Stancheva, “Higher Expression Levels of miR-1246 in Advanced Laryngeal Carcinoma Are Associated with Elevated Risk of Locoregional Metastasis”, C. R. Acad. Bulg. Sci. , vol. 76, no. 3, pp. 464–472, Mar. 2023.

Issue

Section

Medicine