Bacterial and Viral Pathogens Implicated in Female Reproductive Failure Investigated on Menstrual Blood

Authors

  • Kremena Mesechkova Department of Medical Chemistry and Biochemistry, Medical University Sofia and Genetic Medico-diagnostic Laboratory "Genica", Bulgaria
  • Anita Kavrakova Department of Medical Chemistry and Biochemistry, Medical University Sofia and Genetic Medico-diagnostic Laboratory "Genica", Bulgaria
  • Bilyana Georgieva Department of Medical Chemistry and Biochemistry, Medical University Sofia, Bulgaria
  • Ivan Sigridov Dr Sigridov – Medical Center, Bulgaria
  • Ani Miteva Department of Law and Ethics in Medicine, Faculty of Public Health, Medical University Sofia, Bulgaria
  • Vanyo Mitev Department of Medical Chemistry and Biochemistry, Medical University Sofia, Bulgaria
  • Albena Todorova Department of Medical Chemistry and Biochemistry, Medical University Sofia and Genetic Medico-diagnostic Laboratory "Genica", Bulgaria

DOI:

https://doi.org/10.7546/CRABS.2023.03.08

Keywords:

HHVs, infertility, menstrual blood

Abstract

We investigate the impact of bacterial (Chlamydia trachomatis, Ureaplasma urealyticum/parvum, Mycomplasma hominis/genitalium, Gardnerella vaginalis) and viral (HSV1/2, EBV, CMV, VZV, HHV6/HHV7, HHV8) pathogens, as a potential cause of reproductive failure in women by analysis of menstrual blood. We analyzed DNA extracted from 48 probands selected on the basis of history of infertility. DNA extraction, Real-time qPCR, gel electrophoresis were applied.

In 64.6% of all tested menstrual blood samples of infertile women bacterial and/or viral pathogens were detected. In 41.4% of all tested samples we found bacterial, while in 37.5% viral pathogens. Ureaplasma parvum and Gardnerella vaginalis were detected in 58.3% and 54.2%, respectively, of the positive for bacterial pathogens samples. EBV, HHV7 and HHV6 were detected in 38.9%, 55.6%, and, respectively, 11.1% of the positive for viral pathogens samples. Bacterial and viral co-infection was found in 22.6% of all patients.

Chlamydia trachomatis, Mycomplasma hominis/genitalium, Ureaplasma urealyticum, HSV1, HSV2, CMV, VZV and HHV8 were not detected in the menstrual blood samples.

Our study offers new approach for diagnostics of infections in the upper female genital tract by analysis of menstrual blood. The opportunity to detect asymptomatic bacterial and viral infections in female endometrium contributes to reveal the cause for sterility. Our work contributes to clarify the infectious etiology of reproductive failure which is of a great importance for individualized therapy.

Author Biographies

Kremena Mesechkova, Department of Medical Chemistry and Biochemistry, Medical University Sofia and Genetic Medico-diagnostic Laboratory "Genica", Bulgaria

Mailing Address:
Department of Medical Chemistry and Biochemistry,
Medical University Sofia
2 Zdrave St
1431 Sofia, Bulgaria

and 

Genetic Medico-diagnostic Laboratory "Genica"
84 Ami Boué St
1612 Sofia, Bulgaria

E-mail: kremenaslavova@yahoo.com

Anita Kavrakova, Department of Medical Chemistry and Biochemistry, Medical University Sofia and Genetic Medico-diagnostic Laboratory "Genica", Bulgaria

Mailing Address:
Department of Medical Chemistry and Biochemistry,
Medical University Sofia
2 Zdrave St
1431 Sofia, Bulgaria

and 

Genetic Medico-diagnostic Laboratory "Genica"
84 Ami Boué St
1612 Sofia, Bulgaria

E-mail: annita_25@abv.bg

Bilyana Georgieva, Department of Medical Chemistry and Biochemistry, Medical University Sofia, Bulgaria

Mailing Address:
Department of Medical Chemistry and Biochemistry,
Medical University Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: gueorgievab@yahoo.com

Ivan Sigridov, Dr Sigridov – Medical Center, Bulgaria

Mailing Address:
Dr Sigridov – Medical Center,
Mihail Tenev St
1784 Sofia, Bulgaria

E-mail: ivansigridov@gmail.com

Ani Miteva, Department of Law and Ethics in Medicine, Faculty of Public Health, Medical University Sofia, Bulgaria

Mailing Address:
Department of Law and Ethics in Medicine,
Faculty of Public Health,
Medical University Sofia
8 Byalo more St
1527 Sofia, Bulgaria

E-mail: animiteva@abv.bg

Vanyo Mitev, Department of Medical Chemistry and Biochemistry, Medical University Sofia, Bulgaria

Mailing Address:
Department of Medical Chemistry and Biochemistry,
Medical University Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: mitev@medfac.acad.bg

Albena Todorova, Department of Medical Chemistry and Biochemistry, Medical University Sofia and Genetic Medico-diagnostic Laboratory "Genica", Bulgaria

Mailing Address:
Department of Medical Chemistry and Biochemistry,
Medical University Sofia
2 Zdrave St
1431 Sofia, Bulgaria

and 

Genetic Medico-diagnostic Laboratory "Genica"
84 Ami Boué St
1612 Sofia, Bulgaria

E-mail: todorova_albena@abv.bg

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Published

27-03-2023

How to Cite

[1]
K. Mesechkova, “Bacterial and Viral Pathogens Implicated in Female Reproductive Failure Investigated on Menstrual Blood”, C. R. Acad. Bulg. Sci. , vol. 76, no. 3, pp. 394–406, Mar. 2023.

Issue

Section

Biology