DNA-repair Deficient Strains Chlamydomonas reinhardtii as Good Tools for Environmental Toxicology

Authors

  • Maria Dimitrova Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences
  • Daniela Miteva Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences
  • Teodora Todorova Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences
  • Petya Parvanova Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences
  • Maria Yovkova Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences
  • Martin Dimitrov Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences and Faculty of Biology, Sofia University “St. Kliment Ohridski”
  • Stephka Chankova Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences

DOI:

https://doi.org/10.7546/CRABS.2022.02.07

Keywords:

Chlamydomonas reinhardtii, genotype susceptibility, DSBs induction, oxidative stress, zeocin

Abstract

The aim of the present study was to compare stress response of Chlamydomonas reinhardtii strains with different DNA repair capability to zeocin in order to develop a set of endpoints for the revealing of very low levels of xenobiotics. Four C. reinhardtii genotypes – 137C (wild type); CW15 (cell-wallless); UVS-10 (recombination-repair deficient); UVS-14 (mismatch-repair deficient) and different endpoints were applied – micro-colonies survival assay, malondialdehyde (MDA) and intracellular H2O2 quantities, carotenoids content, double-strand DNA breaks (DSBs) induction. Based on the cell survival, strains could be arranged as follows: WT > CW > UVS10 ∼ UVS14. The strongest oxidative stress, measured as the highest MDA and H2O2 contents, was evaluated for UVS-14 genotype. The highest quantity of DSBs induced was measured after zeocin treatment with low doses (2–10 μg/ml) for UVS-10 genotype (P < 0.01). Based on the data reported, a short-term bioassay based on C. reinhardtii with DNA repair-deficient genotypes is proposed to be successfully used in genotoxicity screening of low doses xenobiotics – micro-colonies assay on both strains (UVS-10 and UVS-14); measurement of the kinetics of MDA and H2O2 content on strain UVS-14, and primary induced DSBs levels on strain UVS-10.

Author Biographies

Maria Dimitrova, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences

Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences
2 Gagarin St
1113 Sofia, Bulgaria

mimi.polimenova@gmail.com

Daniela Miteva, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences

Institute of Biodiversity and Ecosystem Research,
Bulgarian Academy of Sciences
2 Gagarin St
1113 Sofia, Bulgaria

daniela.miteva@abv.bg

Teodora Todorova, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences

Institute of Biodiversity and Ecosystem Research,
Bulgarian Academy of Sciences
2 Gagarin St
1113 Sofia, Bulgaria

tedi_todorova@yahoo.com

Petya Parvanova, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences

Institute of Biodiversity and Ecosystem Research,
Bulgarian Academy of Sciences
2 Gagarin St
1113 Sofia, Bulgaria

petq_parvanova@abv.bg

Maria Yovkova, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences

Institute of Biodiversity and Ecosystem Research,
Bulgarian Academy of Sciences
2 Gagarin St
1113 Sofia, Bulgaria

mariya_yovkova@abv.bg

Martin Dimitrov, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences and Faculty of Biology, Sofia University “St. Kliment Ohridski”

Institute of Biodiversity and Ecosystem Research,
Bulgarian Academy of Sciences
2 Gagarin St
1113 Sofia, Bulgaria

Faculty of Biology,
Sofia University “St. Kliment Ohridski”
8 Dragan Tsankov Blvd
1164 Sofia, Bulgaria

mdimitrov83@gmail.com

Stephka Chankova, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences

Institute of Biodiversity and Ecosystem Research,
Bulgarian Academy of Sciences
2 Gagarin St
1113 Sofia, Bulgaria

stephanie.chankova@yahoo.com

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Published

02-03-2022

How to Cite

[1]
M. Dimitrova, “DNA-repair Deficient Strains Chlamydomonas reinhardtii as Good Tools for Environmental Toxicology”, C. R. Acad. Bulg. Sci. , vol. 75, no. 2, pp. 223–229, Mar. 2022.

Issue

Section

Biology