HPLC Determination of Biperiden in Solid Dosage Forms
Keywords:biperiden, HPLC-UV, assay, method development
A rapid, new analytical method, based on liquid chromatography with UV detection, has been developed and utilised for the determination of biperiden hydrochloride in solid dosage forms. The chromatographic separation was performed on a conventional C18 reversed phase column with a mobile phase composed of acetonitrile-buffer mixture. Ultraviolet detection was carried out at 205 nm. A rapid extraction procedure using ultrasonic bath obtained good extraction yield values for the analyte (≥ 98.4%). The method was developed specifically for routine analysis of biperiden in solid dosage forms in the applicable concentration range. The linearity and range of the method investigated for the assay of biperiden is between 2–6 μg mL−1. The method is selective and sufficiently precise. Thus, the method developed is suitable for routine analysis of biperiden.
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LicenseCopyright (c) 2023 Proceedings of the Bulgarian Academy of Sciences
Copyright (c) 2022 Proceedings of the Bulgarian Academy of Sciences
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