HPLC Determination of Biperiden in Solid Dosage Forms

Authors

  • Trifon Milchev Angelov Faculty of Pharmacy, Medical University of Pleven, Bulgaria
  • Biljana Slaveva Krastanova Tchaikapharma High Quality Medicines Inc., Bulgaria
  • Alexandar Svilenov Pashev Faculty of Pharmacy, Medical University of Pleven, Bulgaria
  • Maya Chavdarova Jotova Faculty of Pharmacy, Medical University of Pleven, Bulgaria

DOI:

https://doi.org/10.7546/CRABS.2023.02.02

Keywords:

biperiden, HPLC-UV, assay, method development

Abstract

A rapid, new analytical method, based on liquid chromatography with UV detection, has been developed and utilised for the determination of biperiden hydrochloride in solid dosage forms. The chromatographic separation was performed on a conventional C18 reversed phase column with a mobile phase composed of acetonitrile-buffer mixture. Ultraviolet detection was carried out at 205 nm. A rapid extraction procedure using ultrasonic bath obtained good extraction yield values for the analyte (≥ 98.4%). The method was developed specifically for routine analysis of biperiden in solid dosage forms in the applicable concentration range. The linearity and range of the method investigated for the assay of biperiden is between 2–6 μg mL−1. The method is selective and sufficiently precise. Thus, the method developed is suitable for routine analysis of biperiden.

Author Biographies

Trifon Milchev Angelov, Faculty of Pharmacy, Medical University of Pleven, Bulgaria

Mailing Address:
Faculty of Pharmacy,
Medical University of Pleven
1 St. Kliment Ohridski St
5800 Pleven, Bulgaria

E-mail: t.angelov-mupleven@outlook.com

Biljana Slaveva Krastanova, Tchaikapharma High Quality Medicines Inc., Bulgaria

Mailing Address:
Tchaikapharma High Quality
Medicines Inc.
1 G. M. Dimitrov Blvd
1172 Sofia, Bulgaria

E-mail: b.Krastanova.hq@tchaikapharma.com

Alexandar Svilenov Pashev, Faculty of Pharmacy, Medical University of Pleven, Bulgaria

Mailing Address:
Faculty of Pharmacy,
Medical University of Pleven
1 St. Kliment Ohridski St
5800 Pleven, Bulgaria

E-mail: alexandar.pashev@gmail.com

Maya Chavdarova Jotova, Faculty of Pharmacy, Medical University of Pleven, Bulgaria

Mailing Address:
Faculty of Pharmacy,
Medical University of Pleven
1 St. Kliment Ohridski St
5800 Pleven, Bulgaria

E-mail: m_jotova@mu-pleven.bg

Downloads

Published

26-02-2023

How to Cite

[1]
T. Angelov, B. Krastanova, A. Pashev, and M. Jotova, “HPLC Determination of Biperiden in Solid Dosage Forms”, C. R. Acad. Bulg. Sci. , vol. 76, no. 2, pp. 184–191, Feb. 2023.

Issue

Section

Chemistry