Antibacterial Activity of Combined Nanodiamonds and Snail Fractions with Biocompounds with MW below 10 kDa and above 30 kDa

Authors

  • Elmira Daskalova Faculty of Biology, Sofia University “St. Kliment Ohridski”, Bulgaria
  • Mihaela Belouhova Faculty of Biology, Sofia University “St. Kliment Ohridski” and Center of Competence “Clean technologies for sustainable environment – water, waste, energy for circular economy", Sofia University “St. Kliment Ohridski”, Bulgaria
  • Nellie Zheleva Faculty of Physics, Sofia University “St. Kliment Ohridski” and Center of Competence “Clean technologies for sustainable environment – water, waste, energy for circular economy", Sofia University “St. Kliment Ohridski”
  • Yana Topalova Faculty of Biology,  Sofia University “St. Kliment Ohridski” and Center of Competence “Clean technologies for sustainable environment – water, waste, energy for circular economy", Sofia University “St. Kliment Ohridski”
  • Lyudmila Velkova Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences
  • Aleksandar Dolashki Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences
  • Pavlina Dolashka Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences

DOI:

https://doi.org/10.7546/CRABS.2023.01.04

Keywords:

nanodiamonds, Cornu aspersum, peptides, antibacterial activity, Brevibacillus laterosporus

Abstract

The use of nanomaterials for enhancement of the antibacterial activity of different compounds is a new approach contributing to the efforts of overcoming the emerging antibiotics resistance. This study explores the combined antibacterial activity of nanodiamonds and snail peptides against Brevibacillus laterosporus BT271. Two peptide/protein fractions from Cornu aspersum mucus were used – with molecular weight (MW) below 10 kDa and with MW above 30 kDa. Four types of nanodiamonds (ND) were tested (positively charged, single digit and two types of detonation ND). Peptide fractions were characterized by using MALDI-TOF. The fraction with MW below 10 kDa consisted of many different peptides, while the fraction with proteins above 30 kDa showed proteins at m/z 26879.2 Da, 33903.11 Da, 43583.81 Da, 53986.81 Da, 79091.82 Da, 100650.42 Da, and 141 550.82 Da. When mixture peptides+ND was applied directly on the bacterial culture, the nanodiamonds showed enhanced antibacterial effect from 8% up to 42%. The combination of nanodiamonds and the mucus fraction with MW above 30 kDa had strongest bactericidal effect (1593.04 mm2/mgP*μL). The results demonstrated that ND have potential to enhance the antibacterial activity of the snail peptides but also highlighted that their effects should be carefully studied.

Author Biographies

Elmira Daskalova, Faculty of Biology, Sofia University “St. Kliment Ohridski”, Bulgaria

Mailing Address:
Faculty of Biology,
Sofia University “St. Kliment Ohridski”
8 Dragan Tsankov Blvd
1164 Sofia, Bulgaria

E-mail: baba_emi@abv.bg

Mihaela Belouhova, Faculty of Biology, Sofia University “St. Kliment Ohridski” and Center of Competence “Clean technologies for sustainable environment – water, waste, energy for circular economy", Sofia University “St. Kliment Ohridski”, Bulgaria

Mailing Address:
Faculty of Biology,
Sofia University “St. Kliment Ohridski”
8 Dragan Tsankov Blvd
1164 Sofia, Bulgaria

and

Center of Competence “Clean technologies for sustainable environment – water, waste, energy for circular economy",
Sofia University “St. Kliment Ohridski”
15 Tsar Osvoboditel Blvd
1504 Sofia, Bulgaria

E-mail: mihaela.kirilova@uni-sofia.bg

Nellie Zheleva, Faculty of Physics, Sofia University “St. Kliment Ohridski” and Center of Competence “Clean technologies for sustainable environment – water, waste, energy for circular economy", Sofia University “St. Kliment Ohridski”

Mailing Address:
Faculty of Physics,
Sofia University “St. Kliment Ohridski”
5 James Bourchier Blvd
1164 Sofia, Bulgaria

and

Center of Competence “Clean technologies for sustainable environment – water, waste, energy for circular economy",
Sofia University “St. Kliment Ohridski”
15 Tsar Osvoboditel Blvd
1504 Sofia, Bulgaria

E-mail: zhelevan@phys.uni-sofia.bg

Yana Topalova, Faculty of Biology,  Sofia University “St. Kliment Ohridski” and Center of Competence “Clean technologies for sustainable environment – water, waste, energy for circular economy", Sofia University “St. Kliment Ohridski”

Mailing Address:
Faculty of Biology, 
Sofia University “St. Kliment Ohridski”
8 Dragan Tsankov Blvd
1164 Sofia, Bulgaria

and

Center of Competence “Clean technologies for sustainable environment – water, waste, energy for circular economy",
Sofia University “St. Kliment Ohridski”
15 Tsar Osvoboditel Blvd
1504 Sofia, Bulgaria

E-mail: ytopalova@uni-sofia.bg

Lyudmila Velkova, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences

Mailing Address:
Institute of Organic Chemistry with Centre of Phytochemistry,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 9
1113 Sofia, Bulgaria

E-mail: lyudmila_velkova@abv.bg

Aleksandar Dolashki, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences

Mailing Address:
Institute of Organic Chemistry with Centre of Phytochemistry,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 9
1113 Sofia, Bulgaria

E-mail: adolashki@yahoo.com

Pavlina Dolashka, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences

Mailing Address:
Institute of Organic Chemistry with Centre of Phytochemistry,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 9
1113 Sofia, Bulgaria

E-mail: pda54@abv.bg

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Published

30-01-2023

How to Cite

[1]
E. Daskalova, “Antibacterial Activity of Combined Nanodiamonds and Snail Fractions with Biocompounds with MW below 10 kDa and above 30 kDa”, C. R. Acad. Bulg. Sci. , vol. 76, no. 1, pp. 35–43, Jan. 2023.

Issue

Section

Biology