Secreted Factors from Umbilical Cord-Mesenchymal Stem Cells Affect CD86 Expression on B Lymphocytes and Increase CCL5 Chemokine Secretion by Healthy Donors' PBMC

Authors

  • Adelina Yordanova University Hospital “St. Ivan Rilski”, Laboratory of Clinical Immunology, Department of Clinical Immunology, Medical Faculty, Medical University of Sofia, Bulgaria
  • Dobroslav Kyurkchiev University Hospital “St. Ivan Rilski”, Laboratory of Clinical Immunology, Department of Clinical Immunology, Medical Faculty, Medical University of Sofia, Bulgaria
  • Mariana Ivanova University Hospital “St. Ivan Rilski”, Clinic of Rheumatology, Department of Rheumatology, Medical Faculty, Medical University of Sofia, Bulgaria
  • Kalina Tumangelova-Yuzeir University Hospital “St. Ivan Rilski”, Laboratory of Clinical Immunology, Department of Clinical Immunology, Medical Faculty, Medical University of Sofia, Bulgaria
  • Kalina Belemezova Department of Biology, Medical Faculty, Medical University of Sofia, Bulgaria
  • Ekaterina Ivanova-Todorova University Hospital “St. Ivan Rilski”, Laboratory of Clinical Immunology, Department of Clinical Immunology, Medical Faculty, Medical University of Sofia, Bulgaria

DOI:

https://doi.org/10.7546/CRABS.2022.11.17

Keywords:

mesenchymal stem cells, B lymphocytes, CD86, CCL5

Abstract

A growing number of autoimmune diseases, allergies, and atopic conditions in recent years are calling for the development of innovative therapies, such as those using stem cells with immunoregulatory activity. Because of the few and often conflicting results in the scientific literature, it is still uncertain whether mesenchymal stem cells (MSC) could be a useful therapeutic strategy for targeting B lymphocytes and be helpful in treating autoimmune and allergy patients or those with a variety of viral infections. In connection with the aforementioned, our team investigated the influence of umbilical cord MSC-secreted factors on healthy donors' B-cell CD86 expression and CCL5 secretion by peripheral blood mononuclear cells (PBMC) in vitro. When healthy donors' PBMC were cultivated with conditioned medium derived from umbilical cord MSC, CD86 density on the B-cell membrane statistically decreased, but the percentage of B cells expressing this co-stimulatory molecule was slightly but significantly increased. We also found significantly increased secretion of the chemokine CCL5 by PBMC under the influence of the MSC secretome.

Author Biographies

Adelina Yordanova, University Hospital “St. Ivan Rilski”, Laboratory of Clinical Immunology, Department of Clinical Immunology, Medical Faculty, Medical University of Sofia, Bulgaria

Mailing Address:
University Hospital “St. Ivan Rilski”,
Laboratory of Clinical Immunology,
Department of Clinical Immunology,
Medical Faculty,
Medical University of Sofia, Bulgaria
15 Akademik Iv. E. Geshov Blvd
1431 Sofia, Bulgaria

Email: adi.7@abv.bg

Dobroslav Kyurkchiev, University Hospital “St. Ivan Rilski”, Laboratory of Clinical Immunology, Department of Clinical Immunology, Medical Faculty, Medical University of Sofia, Bulgaria

Mailing Address:
University Hospital “St. Ivan Rilski”,
Laboratory of Clinical Immunology,
Department of Clinical Immunology,
Medical Faculty,
Medical University of Sofia, Bulgaria
15 Akademik Iv. E. Geshov Blvd
1431 Sofia, Bulgaria

Email: dkyurkchiev@medfac.mu-sofia.bg

Mariana Ivanova, University Hospital “St. Ivan Rilski”, Clinic of Rheumatology, Department of Rheumatology, Medical Faculty, Medical University of Sofia, Bulgaria

Mailing Address:
University Hospital “St. Ivan Rilski”,
Clinic of Rheumatology,
Department of Rheumatology,
Medical Faculty,
Medical University of Sofia, Bulgaria
13 Urvich St
1612 Sofia, Bulgaria

E-mail: mariana_ig@abv.bg

Kalina Tumangelova-Yuzeir, University Hospital “St. Ivan Rilski”, Laboratory of Clinical Immunology, Department of Clinical Immunology, Medical Faculty, Medical University of Sofia, Bulgaria

Mailing Address:
University Hospital “St. Ivan Rilski”,
Laboratory of Clinical Immunology,
Department of Clinical Immunology,
Medical Faculty,
Medical University of Sofia, Bulgaria
15 Akademik Iv. E. Geshov Blvd
1431 Sofia, Bulgaria

Email: ktuzeir@medfac.mu-sofia.bg

Kalina Belemezova, Department of Biology, Medical Faculty, Medical University of Sofia, Bulgaria

Mailing Address:
Department of Biology,
Medical Faculty,
Medical University of Sofia, Bulgaria
Ob/Gyn Hospital Dr Shterev, Sofia, Bulgaria
25 Hristo Blagoev St
1330 Sofia, Bulgaria

E-mail: kalina.belemezova@gmail.com

Ekaterina Ivanova-Todorova, University Hospital “St. Ivan Rilski”, Laboratory of Clinical Immunology, Department of Clinical Immunology, Medical Faculty, Medical University of Sofia, Bulgaria

Mailing Address:
University Hospital “St. Ivan Rilski”,
Laboratory of Clinical Immunology,
Department of Clinical Immunology,
Medical Faculty,
Medical University of Sofia, Bulgaria
15 Akademik Iv. E. Geshov Blvd
1431 Sofia, Bulgaria

Email: etodorova@medfac.mu-sofia.bg

Downloads

Published

30-11-2022

How to Cite

[1]
A. Yordanova, D. Kyurkchiev, M. Ivanova, K. Tumangelova-Yuzeir, K. Belemezova, and E. Ivanova-Todorova, “Secreted Factors from Umbilical Cord-Mesenchymal Stem Cells Affect CD86 Expression on B Lymphocytes and Increase CCL5 Chemokine Secretion by Healthy Donors’ PBMC”, C. R. Acad. Bulg. Sci. , vol. 75, no. 11, pp. 1687–1695, Nov. 2022.

Issue

Section

Medicine