Research of the Genetic Diversity of TT Viruses in Bulgaria

Authors

  • Kalina Shishkova Laboratory of Virology, Faculty of Biology, Sofia University “St. Kliment Ohridski", Bulgaria
  • Iliya Tsekov University Multidisciplinary Hospital for Active Treatment “Sofiamed", Bulgaria
  • Rumen Popov Centre for Transfusion Haematology, Military Medical Academy, Bulgaria
  • Stoyan Shishkov Laboratory of Virology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, Bulgaria
  • Zlatko Kalvatchev Multidisciplinary Hospital for Active Treatment “Nadezhda”, Bulgaria

DOI:

https://doi.org/10.7546/CRABS.2022.11.16

Keywords:

TT virus, sequence analysis, TTV prevalence

Abstract

Torque teno viruses (TTVs) are a member of the newly formed family Anelloviridae and contain single stranded circular DNA genome. It is believed that these viruses chronically infect people of any age, yet it is not clear what is their pathogenic potential. Our previous research has shown spread of TT viruses at least 61.87% in voluntary blood donors, 62.74% in patients with respiratory diseases, 65.21% in cases of hepatitis, 62.96% in unknown cases of pathology, 66.66% in patients with brain tumour and 64.28% in patients with renal transplantation. It was the first report demonstrating TT viruses in Bulgaria. The purpose of this work is identification of the main genotypes of TT viruses circulating in Bulgaria. The original viral DNA was isolated from blood samples from clinically healthy blood donors, a HBsAg positive sample and a patient with unexplained febrile condition. Phylogenetic tree analysis shows that most of our sequences are genetically related, highly correlated, and fall into a single cluster. The analysis shows that they are close relatives and most likely have a common ancestor. Part of the sequences could also be a separate cluster or the start of a new clone within that cluster, together with the reference sequences clustered by literature data in genotype 1.

Author Biographies

Kalina Shishkova, Laboratory of Virology, Faculty of Biology, Sofia University “St. Kliment Ohridski", Bulgaria

Mailing Address:
Laboratory of Virology,
Faculty of Biology,
Sofia University “St. Kliment Ohridski”
8 Dragan Tsankov Blvd
1164 Sofia, Bulgaria

E-mail: k_shishkova@biofac.uni-sofia.bg

Iliya Tsekov, University Multidisciplinary Hospital for Active Treatment “Sofiamed", Bulgaria

Mailing Address:
University Multidisciplinary Hospital
for Active Treatment “Sofiamed”
16 G. M. Dimitrov Blvd
1797 Sofia, Bulgaria

E-mail: tsekovmd@gmail.com

Rumen Popov, Centre for Transfusion Haematology, Military Medical Academy, Bulgaria

Mailing Address:
Centre for Transfusion Haematology,
Military Medical Academy
3 St. Georgi Sofiiski St
1606 Sofia, Bulgaria

E-mail: popov1rumen@gmail.com

Stoyan Shishkov, Laboratory of Virology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, Bulgaria

Mailing Address:
Laboratory of Virology,
Faculty of Biology,
Sofia University “St. Kliment Ohridski”
8 Dragan Tsankov Blvd
1164 Sofia, Bulgaria

E-mail: sshishkov@biofac.uni-sofia.bg

Zlatko Kalvatchev, Multidisciplinary Hospital for Active Treatment “Nadezhda”, Bulgaria

Mailing Address:
Multidisciplinary Hospital for Active Treatment “Nadezhda”
3 Blaga Vest St
1330 Sofia, Bulgaria

E-mail: kalvatchev@gmail.com

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Published

30-11-2022

How to Cite

[1]
K. Shishkova, I. Tsekov, R. Popov, S. Shishkov, and Z. Kalvatchev, “Research of the Genetic Diversity of TT Viruses in Bulgaria”, C. R. Acad. Bulg. Sci. , vol. 75, no. 11, pp. 1680–1686, Nov. 2022.

Issue

Section

Medicine