The Endogenous Cannabinoid System and Nitric Oxide Interact in Modulation of Cold Stress-induced Analgesia

Authors

  • Hristina Nocheva Department of Physiology and Pathophysiology, Faculty of Medicine, Medical University – Sofia, Bulgaria
  • Eleonora N. Encheva-Stoykova Department of Physiology and Pathophysiology, Faculty of Medicine, Medical University – Sofia, Bulgaria
  • Georgi Bogdanov Department of Pharmacology and Toxicology, Faculty of Medicine, Medical University – Sofia, Bulgaria
  • Roman Tashev Department of Physiology and Pathophysiology, Faculty of Medicine, Medical University – Sofia, Bulgaria
  • Rumen Nikolov Department of Pharmacology and Toxicology, Faculty of Medicine, Medical University – Sofia, Bulgaria

DOI:

https://doi.org/10.7546/CRABS.2022.11.15

Keywords:

endocannabinoids, nitric oxide, nociception, paw-pressure test

Abstract

The aim of the present study was to estimate 1) whether the endogenous cannabinoid and the nitric oxide-ergic systems' interaction affects cold stress-induced analgesia (c-SIA), and 2) whether the possible interaction (and its impact on c-SIA) differs before and after cold-stress exposure.

Male Wistar rats have been injected with CB1-receptor agonist anandamide (AEA) and the nitric oxide (NO) precursor L-arginine before and after 1 hour of cold stress (1 h CS) exposure; CB1 antagonist AM251, the nitric oxide synthase inhibitor L-NAME, and the NO-donor SIN-1 were additionally applied in order to further elucidate the interaction between the two systems.

Results obtained suggest that endocannabinoids and NO differently interact before and after stress: applied before stress, the two systems were antagonistic between them with AEA exerting an analgesic effect, while NO decreased analgesia; a synergistic effect of endocannabinoids and NO resulted instead in application after 1 h CS.

Author Biographies

Hristina Nocheva, Department of Physiology and Pathophysiology, Faculty of Medicine, Medical University – Sofia, Bulgaria

Mailing Address:
Department of Physiology and Pathophysiology,
Faculty of Medicine,
Medical University – Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: hndimitrova@medfac.mu-sofia.bg

Eleonora N. Encheva-Stoykova, Department of Physiology and Pathophysiology, Faculty of Medicine, Medical University – Sofia, Bulgaria

Mailing Address:
Department of Physiology and Pathophysiology,
Faculty of Medicine,
Medical University – Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: eencheva@medfac.mu-sofia.bg

Georgi Bogdanov, Department of Pharmacology and Toxicology, Faculty of Medicine, Medical University – Sofia, Bulgaria

Mailing Address:
Department of Pharmacology and Toxicology,
Faculty of Medicine,
Medical University – Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: dr.georgi.bogdanov@gmail.com

Roman Tashev, Department of Physiology and Pathophysiology, Faculty of Medicine, Medical University – Sofia, Bulgaria

Mailing Address:
Department of Physiology and Pathophysiology,
Faculty of Medicine,
Medical University – Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: romantashev@gmail.com

Rumen Nikolov, Department of Pharmacology and Toxicology, Faculty of Medicine, Medical University – Sofia, Bulgaria

Mailing Address:
Department of Pharmacology and Toxicology,
Faculty of Medicine,
Medical University – Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: rumen8684@gmail.com

Downloads

Published

30-11-2022

How to Cite

[1]
H. Nocheva, E. Encheva-Stoykova, G. Bogdanov, R. Tashev, and R. Nikolov, “The Endogenous Cannabinoid System and Nitric Oxide Interact in Modulation of Cold Stress-induced Analgesia”, C. R. Acad. Bulg. Sci. , vol. 75, no. 11, pp. 1672–1679, Nov. 2022.

Issue

Section

Medicine