Quercetin Influences Phospholipase A2 Products in A549 Adenocarcinoma Cells

Authors

  • Biliana Nikolova Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences
  • Roumen Pankov Biological Faculty, Sofia University ``St. Kliment Ohridski''
  • Galya Staneva Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences
  • Nikolai Krastev Department of Anatomy, Histology and Embryology, Medical University – Sofia, Bulgaria
  • Tania Markovska Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences
  • Stefan Pankov Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences
  • Evgenia Vassileva Clinic of Neurology, Tsaritsa Yoanna University Hospital-ISUL, Bulgaria
  • Dimo Krastev Medical University – Sofia, Medical College “Y. Filaretova”, Bulgaria
  • Albena Momchilova Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

DOI:

https://doi.org/10.7546/CRABS.2022.11.05

Keywords:

quercetin, phosphatidylcholine, phospholipase A2, arachidonic acid, lipid peroxides

Abstract

The effect of quercetin on phospholipase A2 activity and its products has been investigated in A549 adenocarcinoma cells. Quercetin is a polyphenol of medico-biological significance, which has been reported to show antioxidant and anti-tumour beneficial effects. Phospholipids are functionally active molecules, which participate in various cellular processes, acting as second messengers, enzyme activators, membrane transporters, etc. Although there are many studies dealing with the influence of quercetin on the lipid metabolism of different types of cancer cells, the mechanism of this effect

remains largely unclear. The present study showed that quercetin treatment of A549 cells induced activation of phospholipase A2 (PLA2) activity, the latter being known as a source of pro-inflammatory intermediate metabolites. The phospholipid analysis of membranes from A549 cells revealed reduction of

phosphatidylcholine (PC) and an increase of the level of lysophosphatidylcholine (LPC). In addition, acyl chain analysis showed an elevation of some polyunsaturated fatty acids, such as arachidonic acid (AA), the latter being a major product of PLA2. The content of lipid peroxides was also elevated in membranes from quercetin-treated A549 cells, which we presume is related to the higher content of polyunsaturated fatty acids, which are an excellent target of oxidative destruction.

In conclusion, the present results show that treatment of A549 adenocarcinoma cells with the polyphenol quercetin induces activation of membrane-bound PLA2, which is a source polyunsaturated fatty acids, the

latter producing lipid peroxides. We presume that this mechanism underlies the reported by other authors elevation of oxidative stress, which is induced by quercetin treatment of cancerous cells.

Author Biographies

Biliana Nikolova, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

Mailing Address:
Institute of Biophysics and Biomedical Engineering,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: nikolova@bio21.bas.bg

Roumen Pankov, Biological Faculty, Sofia University ``St. Kliment Ohridski''

Mailing Address:
Biological Faculty,
Sofia University ``St. Kliment Ohridski''
8 Dragan Tzankov Blvd
1164 Sofia, Bulgaria

E-mail: rpankov@biofac.uni-sofia.bg

Galya Staneva, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

Mailing Address:
Institute of Biophysics and Biomedical Engineering,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: g_staneva@yahoo.com

Nikolai Krastev, Department of Anatomy, Histology and Embryology, Medical University – Sofia, Bulgaria

Mailing Address:
Department of Anatomy, Histology and Embryology,
Medical University – Sofia
1 St. Georgi Sofiiski Blvd
1431 Sofia, Bulgaria

E-mail: dr.krustev.dm@gmail.com

Tania Markovska, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

Mailing Address:
Institute of Biophysics and Biomedical Engineering,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: Markobska@abv.bg

Stefan Pankov, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

Mailing Address:
Institute of Biophysics and Biomedical Engineering,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: stefan.pankov@abv.bg

Evgenia Vassileva, Clinic of Neurology, Tsaritsa Yoanna University Hospital-ISUL, Bulgaria

Mailing Address:
Clinic of Neurology,
Tsaritsa Yoanna University Hospital-ISUL
8 Byalo more St
1527 Sofia, Bulgaria

E-mail: e.vassilevva@gmail.com

Dimo Krastev, Medical University – Sofia, Medical College “Y. Filaretova”, Bulgaria

Mailing Address:
Medical University – Sofia,
Medical College “Y. Filaretova”
3 Yordanka Filaretova St
1606 Sofia, Bulgaria

E-mail: Dimo_krustev@mail.bg

Albena Momchilova, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

Mailing Address:
Institute of Biophysics and Biomedical Engineering,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: albena_momchilova@abv.bg

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Published

30-11-2022

How to Cite

[1]
B. Nikolova, “Quercetin Influences Phospholipase A2 Products in A549 Adenocarcinoma Cells”, C. R. Acad. Bulg. Sci. , vol. 75, no. 11, pp. 1588–1594, Nov. 2022.

Issue

Section

Biology