Study on the Relationship Between Male Infertility and DNA Integrity in Spermatozoa Nuclei

Authors

  • Vesela Mitkovska Biological Faculty - University of Plovdiv “Paisii Hilendarski”
  • Spas Dzhoglov Biological Faculty - University of Plovdiv “Paisii Hilendarski”
  • Doychin Boyadzhiev Faculty of Mathematics and Informatics - University of Plovdiv “Paisii Hilendarski”
  • Penka Vasileva Biological Faculty - University of Plovdiv “Paisii Hilendarski”
  • Ivan Stoyanov Biological Faculty - University of Plovdiv “Paisii Hilendarski”
  • Teodora Popova Biological Faculty - University of Plovdiv “Paisii Hilendarski”
  • Teodora Staykova Biological Faculty - University of Plovdiv “Paisii Hilendarski”
  • Evgeniya N. Ivanova Biological Faculty - University of Plovdiv “Paisii Hilendarski”

DOI:

https://doi.org/10.7546/CRABS.2022.01.14

Keywords:

male infertility, semen quality, sperm DNA fragmentations

Abstract

The purpose of the present study is to assess the potential relationships between the diagnoses for male infertility based on conventional sperm analysis and the levels of sperm nuclei with damaged DNA in men with reproductive problems using the acridine orange fluorescent test. Totally 87 men with reproductive problems have been investigated. Excellent and good fertility potential was established for 58.6% of the studied individuals, and fair and poor potential of male fertility – for 41.4%. Different diagnoses have been found for the men included in the study – normosoospermia (58.6%), asthenozoospermia (18.4%), oligoasthenozoospermia (11.5%), oligoasthenoteratozoospermia (9.2%) and asthenoteratozoospermia (2.3%). Statistically significant dependencies (P < 0.001) have been observed between the studied sperm quality parameters, as well as for infertility diagnoses done and the level of spermatozoa nuclei with DNA damage. The present study suggests that the acridine orange fluorescence test is sufficiently informative to determine the quality of the fertility potential. The obtained results could be used as a basis for future detailed research on male reproductive health and the potential risks for its deterioration.

Author Biographies

Vesela Mitkovska, Biological Faculty - University of Plovdiv “Paisii Hilendarski”

Department of Developmental Biology
Biological Faculty
University of Plovdiv “Paisii Hilendarski”
24, Tsar Asen St
4000 Plovdiv, Bulgaria
mitkovska.v@gmail.com

Spas Dzhoglov, Biological Faculty - University of Plovdiv “Paisii Hilendarski”

Department of Developmental Biology
Biological Faculty
University of Plovdiv “Paisii Hilendarski”
24, Tsar Asen St
4000 Plovdiv, Bulgaria
spas78dj@abv.bg

Doychin Boyadzhiev, Faculty of Mathematics and Informatics - University of Plovdiv “Paisii Hilendarski”

Faculty of Mathematics and Informatics
University of Plovdiv “Paisii Hilendarski”
24, Tsar Asen St
4000 Plovdiv, Bulgaria

Penka Vasileva, Biological Faculty - University of Plovdiv “Paisii Hilendarski”

Department of Developmental Biology
Biological Faculty
University of Plovdiv “Paisii Hilendarski”
24, Tsar Asen St
4000 Plovdiv, Bulgaria
pepa_vasileva@abv.bg

Ivan Stoyanov, Biological Faculty - University of Plovdiv “Paisii Hilendarski”

Department of Developmental Biology
Biological Faculty
University of Plovdiv “Paisii Hilendarski”
24, Tsar Asen St
4000 Plovdiv, Bulgaria
ivan100yanov@abv.bg

Teodora Popova, Biological Faculty - University of Plovdiv “Paisii Hilendarski”

Department of Developmental Biology
Biological Faculty
University of Plovdiv “Paisii Hilendarski”
24, Tsar Asen St
4000 Plovdiv, Bulgaria
teodora.irikova@gmail.com

Teodora Staykova, Biological Faculty - University of Plovdiv “Paisii Hilendarski”

Department of Developmental Biology
Biological Faculty
University of Plovdiv “Paisii Hilendarski”
24, Tsar Asen St
4000 Plovdiv, Bulgaria
tstaykova@yahoo.com

Evgeniya N. Ivanova, Biological Faculty - University of Plovdiv “Paisii Hilendarski”

Department of Developmental Biology
Biological Faculty
University of Plovdiv “Paisii Hilendarski”
24, Tsar Asen St
4000 Plovdiv, Bulgaria
e.ivanova@gmail.com

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Published

02-02-2022

How to Cite

[1]
V. Mitkovska, “Study on the Relationship Between Male Infertility and DNA Integrity in Spermatozoa Nuclei ”, C. R. Acad. Bulg. Sci. , vol. 75, no. 1, pp. 121–128, Feb. 2022.

Issue

Section

Medicine