The Verification of Differentially Expressed Genes Related to Feather Colour in Quail Embryos by RT-qPCR Based on RNA-seq

Authors

  • Yunqi Hu College of Animal Science, Henan University of Science and Technology
  • Xiaohui Zhang College of Animal Science, Henan University of Science and Technology and Luoyang Key Laboratory of Animal Genetic and Breeding
  • Youzhi Pang College of Animal Science, Henan University of Science and Technology and Luoyang Key Laboratory of Animal Genetic and Breeding
  • Yanxia Qi College of Animal Science, Henan University of Science and Technology and Luoyang Key Laboratory of Animal Genetic and Breeding
  • Qiankun Wang College of Animal Science, Henan University of Science and Technology, China
  • Yiwei Zhao College of Animal Science, Henan University of Science and Technology, China
  • Zhiwen Yuan College of Animal Science, Henan University of Science and Technology, China
  • Tao Wang College of Animal Science, Henan University of Science and Technology, China
  • Linke Huo College of Animal Science, Henan University of Science and Technology, China
  • Shiwei Ren College of Animal Science, Henan University of Science and Technology, China

DOI:

https://doi.org/10.7546/CRABS.2022.08.06

Keywords:

quail, RT-qPCR, gene expression, RNA-seq, feather colour

Abstract

Many differentially expressed genes associated with feather colour in quail embryos can be screened using RNA-seq technology. The aim of this study was to verify the accuracy of RNA-seq using RT-qPCR. The transcriptome of embryonic skin tissues of quails were sequenced by Illumina HiSeq TM2000 sequencing platform. As a result, 2512 differentially expressed genes were found. Among them, 950 differentially expressed genes were up-regulated in the skin tissues of white-feather quail embryos, and 1562 differentially expressed genes were down-regulated. The candidate genes were analyzed by RT-qPCR method to verify the accuracy and reliability of the RNA-seq. The results showed that the relative expression levels of ASIP and DDC in white-feather quails were significantly increased, while HOXD1, CTSD, KRT2, MBD2 and MT1 were significantly decreased, which was consistent with the results of RNA-seq. The validation results of the candidate genes can also provide some ideas to explain the difference in feather colour between maroon-feather quails and white-feather quails.

Author Biographies

Yunqi Hu, College of Animal Science, Henan University of Science and Technology

Mailing Address:
College of Animal Science,
Henan University of Science and Technology
Luoyang, Henan 471003, China

E-mail: hyq18438616207@163.com

Xiaohui Zhang, College of Animal Science, Henan University of Science and Technology and Luoyang Key Laboratory of Animal Genetic and Breeding

Mailing Address:
College of Animal Science,
Henan University of Science and Technology
Luoyang, Henan
471003, China

and

Luoyang Key Laboratory
of Animal Genetic and Breeding,
Luoyang, Henan
471003, China

E-mail: 1175552356@qq.com,
zhangxiaohui78@126.com 

Youzhi Pang, College of Animal Science, Henan University of Science and Technology and Luoyang Key Laboratory of Animal Genetic and Breeding

Mailing Address:
College of Animal Science,
Henan University of Science and Technology
Luoyang, Henan
471003, China

and

Luoyang Key Laboratory
of Animal Genetic and Breeding,
Luoyang, Henan
471003, China

E-mail: 1536971128@qq.com,
pyzh2006@126.com

Yanxia Qi, College of Animal Science, Henan University of Science and Technology and Luoyang Key Laboratory of Animal Genetic and Breeding

Mailing Address:
College of Animal Science,
Henan University of Science and Technology
Luoyang, Henan
471003, China

and

Luoyang Key Laboratory
of Animal Genetic and Breeding,
Luoyang, Henan
471003, China

E-mail: 871536045@qq.com,
qyanxia0330@126.com

Qiankun Wang, College of Animal Science, Henan University of Science and Technology, China

Mailing Address:
College of Animal Science,
Henan University of Science and Technology
Luoyang, Henan
471003, China

E-mail: 1902237716@qq.com

Yiwei Zhao, College of Animal Science, Henan University of Science and Technology, China

Mailing Address:
College of Animal Science,
Henan University of Science and Technology
Luoyang, Henan
471003, China

E-mail: 2858279410@qq.com

Zhiwen Yuan, College of Animal Science, Henan University of Science and Technology, China

Mailing Address:
College of Animal Science,
Henan University of Science and Technology
Luoyang, Henan
471003, China

E-mail: 1847090379@qq.com

Tao Wang, College of Animal Science, Henan University of Science and Technology, China

Mailing Address:
College of Animal Science,
Henan University of Science and Technology
Luoyang, Henan
471003, China

E-mail: 1847090379@qq.com

Linke Huo, College of Animal Science, Henan University of Science and Technology, China

Mailing Address:
College of Animal Science,
Henan University of Science and Technology
Luoyang, Henan
471003, China

E-mail: 1847090379@qq.com

Shiwei Ren, College of Animal Science, Henan University of Science and Technology, China

Mailing Address:
College of Animal Science,
Henan University of Science and Technology
Luoyang, Henan
471003, China

E-mail: 1847090379@qq.com

Downloads

Published

31-08-2022

How to Cite

[1]
Y. Hu, “The Verification of Differentially Expressed Genes Related to Feather Colour in Quail Embryos by RT-qPCR Based on RNA-seq”, C. R. Acad. Bulg. Sci., vol. 75, no. 8, pp. 1138–1145, Aug. 2022.

Issue

Section

Biology