UHPLC-HRMS Based Saponin and Flavonoid Profiling of Chenopodium bonus-henricus L. Roots and Prolipase Activity of the Main Saponins

Authors

  • Zlatina Kokanova-Nedialkova Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia
  • Paraskev Nedialkov Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia

DOI:

https://doi.org/10.7546/CRABS.2022.07.06

Keywords:

Chenopodium bonus-henricus, UHPLC-HRMS, saponins, flavonoids, prolipase activity

Abstract

UHPLC-HRMS profiling method was used for a comprehensive study of flavonoid and saponin-rich fractions from the MeOH extract of C. bonushenricus L. roots. Thirty-one compounds, respectively, 16 saponins of five sapogenins (oleanoic acid, 2-hydroxyoleanoic acid, medicagenic acid, bayogenin, and phytolaccagenin), together with 15 flavonoid glycosides of 6-methoxykaempferol, isorhamnetin, spinacetin, and patuletin were detected. Lipase modulatory activities of the main saponins were established by measuring the levels of the released 4-nitrophenol using LC-MS. All saponins exhibited prolipase activity and could find application in the treatment of cachexia.

Author Biographies

Zlatina Kokanova-Nedialkova, Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia

Mailing Address:
Department of Pharmacognosy,
Faculty of Pharmacy,
Medical University of Sofia
2 Dunav St
1000 Sofia, Bulgaria

E-mail: zlatina.kokanova@pharmfac.mu-sofia.bg

Paraskev Nedialkov, Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia

Mailing Address:
Department of Pharmacognosy,
Faculty of Pharmacy,
Medical University of Sofia
2 Dunav St
1000 Sofia, Bulgaria

E-mail: pnedialkov@pharmfac.mu-sofia.bg

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Published

29-07-2022

How to Cite

[1]
Z. Kokanova-Nedialkova and P. Nedialkov, “UHPLC-HRMS Based Saponin and Flavonoid Profiling of Chenopodium bonus-henricus L. Roots and Prolipase Activity of the Main Saponins”, C. R. Acad. Bulg. Sci. , vol. 75, no. 7, pp. 982–991, Jul. 2022.

Issue

Section

Biology