The Utility of Preimplantation Genetic Test in Couples with History of Infertility and Pregnancy Loss

Authors

  • Savina Hadjidekova Department of Medical Genetics, Medical Faculty, Medical University of Sofia and Nadezhda Women’s Health Hospital
  • Slavyana Yaneva Staykova Department of Medical Genetics, Medical Faculty, Medical University of Sofia
  • Rada Staneva Department of Medical Genetics, Medical Faculty, Medical University of Sofia and Nadezhda Women’s Health Hospital
  • Maria Pancheva Nadezhda Women’s Health Hospital
  • Maria Serafimova Nadezhda Women’s Health Hospital
  • Kristina Nikolova Nadezhda Women’s Health Hospital
  • Draga Toncheva Department of Medical Genetics, Medical Faculty, Medical University of Sofia
  • Georgi Stamenov Nadezhda Women’s Health Hospital

DOI:

https://doi.org/10.7546/CRABS.2022.04.14

Keywords:

PGT, IVF, aCGH, NGS

Abstract

Preimplantation genetic test (PGT) is cutting-edge technology for early detection of genetic abnormalities in embryos prior to their implantation in the uterus. PGT prevents the transfer of affected embryos during in vitro fertilization (IVF) procedures and termination of pregnancy. In our study we report the results from 185 couples with history of infertility and pregnancy loss that had undergone IVF procedures with PGT. Trophectoderm biopsy was carried out on 497 blastocyst stage embryos originating from 231 oocyte retrieval cycles. Array-based comparative genomic hybridization (aCGH) and next-generation sequencing (NGS) were subsequently performed. One hundred and ninety-six embryos had balanced profile (40.16%) and 292 embryos showed unbalanced profile (59.84%). Embryo transfer was performed in 109 cases (58.92%) and biochemical pregnancy was reported in 33.03%. Live birth rate was 29.36% and pregnancy loss occurred in 3.67% of cases. Our results show that PGT reduces the number of meaningless transfers, eliminates the trauma of termination of desired pregnancy and possible medical complications. In couples with reproductive failure PGT decreases the risk for pregnancy loss, increases the chance of conceiving with a chromosomally balanced embryo and live birth of a healthy offspring per transfer.

Author Biographies

Savina Hadjidekova, Department of Medical Genetics, Medical Faculty, Medical University of Sofia and Nadezhda Women’s Health Hospital

Mailing Addresses:
Department of Medical Genetics,
Medical Faculty
Medical University of Sofia
2 Zdrave St
1431 Sofia, Bulgaria

and

Nadezhda Women’s Health Hospital
3 Blaga Vest St
1330 Sofia, Bulgaria

E-mail: savinaagova@yahoo.com

Slavyana Yaneva Staykova, Department of Medical Genetics, Medical Faculty, Medical University of Sofia

Mailing Address:
Department of Medical Genetics,
Medical Faculty,
Medical University of Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: sanniya_yan@hotmail.com

Rada Staneva, Department of Medical Genetics, Medical Faculty, Medical University of Sofia and Nadezhda Women’s Health Hospital

Mailing Addresses:
Department of Medical Genetics,
Medical Faculty,
Medical University of Sofia
2 Zdrave St
1431 Sofia, Bulgaria

and

Nadezhda Women’s Health Hospital
3 Blaga Vest St
1330 Sofia, Bulgaria

E-mail: rada.staneva@gmail.com

Maria Pancheva, Nadezhda Women’s Health Hospital

Mailing Address:
Nadezhda Women’s Health Hospital
3 Blaga Vest St
1330 Sofia, Bulgaria

E-mail: marie.pancheva@gmail.com

Maria Serafimova, Nadezhda Women’s Health Hospital

Mailing Address:
Nadezhda Women’s Health Hospital
3 Blaga Vest St
1330 Sofia, Bulgaria

E-mail: m_serafimova86@abv.bg

Kristina Nikolova, Nadezhda Women’s Health Hospital

Mailing Address:
Nadezhda Women’s Health Hospital
3 Blaga Vest St
1330 Sofia, Bulgaria

E-mail: kristinanikolova377@gmail.com

Draga Toncheva, Department of Medical Genetics, Medical Faculty, Medical University of Sofia

Mailing Address:
Department of Medical Genetics,
Medical Faculty,
Medical University of Sofia
2 Zdrave St
1431 Sofia, Bulgaria

E-mail: dragatoncheva@gmail.com

Georgi Stamenov, Nadezhda Women’s Health Hospital

Mailing Address:
Nadezhda Women’s Health Hospital
3 Blaga Vest St
1330 Sofia, Bulgaria

E-mail: georgistamenov@abv.bg

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Published

02-05-2022

How to Cite

[1]
S. Hadjidekova, “The Utility of Preimplantation Genetic Test in Couples with History of Infertility and Pregnancy Loss”, C. R. Acad. Bulg. Sci. , vol. 75, no. 4, pp. 590–595, May 2022.

Issue

Section

Medicine