Oxidized Lipids Control Lipid Order and Phospholipase A2 Activity in Model Membranes

Authors

  • Vesela Yordanova Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences
  • Rusina Hazarosova nstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences
  • Victoria Vitkova Institute of Solid State Physics, Bulgarian Academy of Sciences
  • Aneliya Kostadinova Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences
  • Miglena Angelova Department of Physics Faculty of Sciences and Engineering, Sorbonne University
  • Albena Momchilova Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences
  • Plamen Krastev Cardiology Clinic, University Hospital “St. Ekaterina”, Bulgaria
  • Galya Staneva Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

DOI:

https://doi.org/10.7546/CRABS.2022.04.13

Keywords:

oxidized lipids, lipid order, phospholipase A2 activity

Abstract

Oxidative stress is an important etiologic factor in the pathogenesis of various diseases. The formation of oxidized phospholipid species in vivo induces membrane remodelling with direct pathological implications with a prominent inflammatory component. Secretory phospholipases A2 (sPLA2) are involved in the regulation of inflammation and immune response. Their activity is highly dependent on the lipid membrane composition, structure and organization. In this work, we studied the impact of oxidized phosphatidylcholines (OxPCs) on the membrane lipid order and the sPLA2 activity. The effects of two of the most physiologically active OxPCs, 1-palmitoyl-2-(5′-oxo-valeroyl)-sn-glycero- 3-phosphocholine (POVPC) and 1-palmitoyl-2-glutaroyl-sn-glycero-3- phosphocholine (PGPC) were compared using 1-palmitoyl-2-oleoyl-sn-glycero- 3-phosphocholine (POPC) vesicles. Both OxPCs reduce the membrane lipid order and sPLA2 activity at physiological temperature. Moreover, these changes depend on the chemical nature of the oxidized chains.

Author Biographies

Vesela Yordanova, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

Mailing Address:
Institute of Biophysics
and Biomedical Engineering,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mails: v.v.yordanova.bul@abv.bg

Rusina Hazarosova, nstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

Mailing Address:
Institute of Biophysics
and Biomedical Engineering,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: r_hazarosova@abv.bg

Victoria Vitkova, Institute of Solid State Physics, Bulgarian Academy of Sciences

Mailing Address:
Institute of Solid State Physics,
Bulgarian Academy of Sciences
72 Tsarigradsko Shosse
1784 Sofia, Bulgaria

E-mail: victoria@issp.bas.bg

Aneliya Kostadinova, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

Mailing Address:
Institute of Biophysics
and Biomedical Engineering,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: aneliakk@yahoo.com

Miglena Angelova, Department of Physics Faculty of Sciences and Engineering, Sorbonne University

Mailing Address:
Department of Physics
Faculty of Sciences and Engineering,
Sorbonne University
Mati`ere et Syst`emes Complexes
(MSC) CNRS UMR 7057
University of Paris Diderot
75013 Paris, France

E-mail: miglena.anguelova@upmc.fr

Albena Momchilova, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

Mailing Address:
Institute of Biophysics
and Biomedical Engineering,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: albena_momchilova@abv.bg

Plamen Krastev, Cardiology Clinic, University Hospital “St. Ekaterina”, Bulgaria

Mailing Address:
Cardiology Clinic,
University Hospital “St. Ekaterina”
52A P. Slaveykov Blvd
1431 Sofia, Bulgaria

E-mail: plamenkr@mail.bg

Galya Staneva, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences

Mailing Address:
Institute of Biophysics
and Biomedical Engineering,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: gstaneva@obzor.bio21.bas.bg

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Published

02-05-2022

How to Cite

[1]
V. Yordanova, “Oxidized Lipids Control Lipid Order and Phospholipase A2 Activity in Model Membranes”, C. R. Acad. Bulg. Sci. , vol. 75, no. 4, pp. 581–589, May 2022.

Issue

Vol. 75 No. 4 (2022)

Section

Medicine

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